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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong, F. Ann Ran, David Cox, Shuailiang Lin - Science - 2013Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by van der Oost ). Cong et al. (p. 819 , published online 3 January) and Mali et al. (p. 823 , published onl…被引用次数：15,373Genome engineering using the CRISPR-Cas9 system
F. Ann Ran, Patrick D. Hsu, Jason Wright, Vineeta Agarwala - Nature Protocols - 2013该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：11,402The new frontier of genome engineering with CRISPR-Cas9
Jennifer A. Doudna, Emmanuelle Charpentier - Science - 2014The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark…被引用次数：6,676CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Rodolphe Barrangou, Christophe Fremaux, Hélène Deveau, Melissa Richards - Science - 2007Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, to…被引用次数：6,150Development and Applications of CRISPR-Cas9 for Genome Engineering
Patrick D. Hsu, Eric S. Lander, Feng Zhang - Cell - 2014该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：5,718Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem, Neville E. Sanjana, Ella Hartenian, Xi Shi - Science - 2013Improving Whole-Genome Screens Improved methods are needed for the knockout of individual genes in genome-scale functional screens. Wang et al. (p. 80 , published online 12 December) and Shalem et al. (p. 84 , published online 12 December) used the bacterial CRISPR/Cas9 system to power-screen protocols that avoid several of the pitfalls associated with small interfering RNA (siRNA) screens. Genome editing by these me…被引用次数：5,356Nucleic acid detection with CRISPR-Cas13a/C2c2
Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler - Science - 2017Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a with recombinase polymerase amplification methods to detect specific RNA and DNA sequences. The method successfully detected attomolar levels of Zika virus, as well as the presen…被引用次数：3,573Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Lei S. Qi, Matthew H. Larson, Luke A. Gilbert, Jennifer A. Doudna - Cell - 2013该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：5,022Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Bernd Zetsche, Jonathan S. Gootenberg, Omar O. Abudayyeh, Ian M. Slaymaker - Cell - 2015该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：4,689Improved vectors and genome-wide libraries for CRISPR screening
Neville E. Sanjana, Ophir Shalem, Feng Zhang - Nature Methods - 2014该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：5,318